Spontaneous and Fertilization-Induced Ca2+ Oscillations in Mouse Immature Germinal Vesicle-Stage Oocytes1
نویسندگان
چکیده
منابع مشابه
Germinal vesicle transfer between fresh and cryopreserved immature mouse oocytes.
BACKGROUND We assessed the maturational competence and the chromosomal pattern of mouse oocytes reconstructed by germinal vesicle (GV) transfer technique using nuclear and/or cytoplasmic components from cryopreserved GV stage oocytes. METHODS From 657 GV oocytes (326 fresh and 331 frozen/thawed), four groups of reconstructed oocytes were obtained by micromanipulation and electrofusion: fresh ...
متن کاملP-29: Effects of Cumulus Cells on Survival, In vitro Maturation,Fertilization and Subsequent Developmental Capacity of Mouse Germinal Vesicle Stage Oocytes during Vitrification
Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...
متن کاملthe effect of retinoic acid on in vitro maturation and fertilization rate of mouse germinal vesicle stage oocytes
objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...
متن کاملThe Effect of Retinoic Acid on in vitro Maturation and Fertilization Rate of Mouse Germinal Vesicle Stage Oocytes
OBJECTIVE Retinoids are recognized as important regulators of cell differention and tissue function. Previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. In this study, we investigated the effect of all-trans retinoic acid (t-RA) on maturation and fertilization rate of immature oocytes (germinal vesicle). MATERIALS AND METHODS G...
متن کاملthe effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1998
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod58.3.807